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PSF distiller SFP volume ... Time series Huygens Essential is able to deconvolve time se-ries of 3D or 2D images, automatically correcting for bleaching and varying backgrounds. Cover illustration and bottom right image: isolated Rat Hepatocyte couplet recorded at the Department of Anatomy, University of Basel, Switzerland (head: Prof. Lukas.

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7.2 Huygens™ Principle I, §1, §3 The spherical wave is the basic wave for light propagation using Huygens™ principle. In 1678, Christiaan Huygens theorized a model for light propagation that claimed that each point on a propagating wavefront (regardless of fishapefl) could be assumed to be a source of a new spherical wave.

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3D Deconvolution Microscopy. The deconvolution is an image-processing technique that restores the effective specimen representation for a 3D microscopy images. Various software packages for deconvolution are available, both commercial ones and open-source ones. They are computationally extensive requiring high-end processors and huge memory.

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